Siderophore detection

Aim

Once our bacteria is transformed with the plasmid with the Fur Binding Site and Lac I and the two plasmids with Enterobactins genes, we need to check if our system really work. The production of siderophores can be detected visualy with Blue Agar Chrome Azurol S (CAS). Without siderophore in the medium, CAS and Hexadecyltrimethylammonium bromide (HDTMA)complexes with ferric iron, producing a blue color. When a bacteria strain produce siderophore, the medium color change from blue to orange.

Preparation