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From 2013.igem.org

NanoDrop data of the miniprepped, defrosted DNA:

The NanoDrop machine was zero-ed using the elution buffer from the gel extraction kit used.

• OmpF A - 20.1 ng/ul
• OmpF B - 9.3ng/ul
• B0034 - 26.7ng/ul
• RFP - 198ng/ul

The concentration for OmpF B was too low, so we continued on to the digestion with only OmpF A. The concentration for RFP was too high, so we diluted it.

• RFP (dilution) - 50.4ng/ul

Digestion

Part	OmpF (Part A)	B0034 (Part B)	AMP Plasmid	RFP (Control)
DNA	12.4 ul	9.4 ul	10.0 ul	5.0 ul
Non-nuclease Water	3.6 ul	6.6 ul	5.5 ul	11.0 ul
NEB Buffer	2.5 ul	2.5 ul	2.5 ul	2.5 ul
BSA	0.5 ul	0.5 ul	0.5 ul	0.5 ul
EcoR1	0.5 ul	-	0.5 ul	0.5 ul
Spe1	0.5	-	-	-
Xbal1	-	0.5 ul	-	-
Pst1	-	0.5 ul	0.5 ul	0.5 ul
Dpn1	-	-	0.5 ul	-