Georgia State/15 May 2013

5/15 Group B: Restriction digest Group C: Made eight hour cultures from plates made 5/13; made YPDS plates (no antibiotics); plated P. pastoris on YPDS plates; made YPD broth; made overnight culture of P. pastoris in YPD from glycerol stock; made overnight culture of E. coli and pGAPzα C from eight hour culture.