Team:Paris Bettencourt/Notebook/TB-ception/Wednesday 21st August.html

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TB-ception

Wednesday 21st August


Our macrophages arrived today and we are staring a cell culture! The cells with pET21b and pSB3C5 can't survive the induction with 1 mM IPTG because they have two IPTG inducable promoters. We want to use a different insert, one with an uninducable promoter, such as BBa_J23100-118.

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