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Contents 1 June 1.1 6/10/13 1.2 6/12/13 1.3 6/14/13 1.4 6/17/13 1.5 6/19/13 1.6 6/21/13

June

6/10/13

Today we looked at a paper describing efficient ways to propagate large phage. They suggested agarose instead of agar, and at a concentration of 0.15% which is 1.5 g/L. Dr. Grose explained to us that agarose is more stable and forms larger pores, requiring a smaller amount of powder to produce a solid, stable gel. LB Agarose top agar can be plated onto normal LB bottom agar. We will make up some agarose top agar and see what size of plaques we get.

6/12/13

6/14/13

6/17/13

6/19/13

6/21/13