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Team:Groningen/Labwork/6 September 2013
From 2013.igem.org
Claudio
The plates (pSB1C3-S1-S5 and pSB1C3-S2-S5) show single colonies.ColonyPCR was performed on 6 colonies per plate (total of 12 colonies) beside one positive control (pSB1C3-S1).
The primers used were VF2 and VR (annealing temperature 56°C).
The samples were checked on agarose gel 0.8%.
pSB1C3-S1-S5 colonies.
pSB1C3-S2-S5 colonies.
Two colonies seem to be positive candidates.
Colony A from the plate pSB1C3-S1-S5 was inoculated over-night in liquid culture (LB + Cm).
Colony B from the plate pSB1C3-S2-S5 was inoculated over-night in liquid culture (LB + Cm).
iGEM 2013 Groningen
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