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From 2013.igem.org

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General Ligation Protocol

Reaction Mixture: 7uL insert (1:3 to 1:7 ratio of vector:insert) & (2:6) also works well
1uL vector
1uL T4 ligase buffer
1uL T4 ligase
10ul total

Incubate at 16C overnight, or room temperature for 30 minutes to 1 hour.

Heat inactivation after ligation, before transformation can increase transformation efficiency.
65C for 10 minutes (or 80C)

DNA ligation with T4 DNA Ligase