09/09/13

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Preparation of overnight broth

  • Following on from 06/09/13 result,overnight broth was prepared yesterday from the single colonies that had grown on each of the 2 plates shown on 06/09/13.


Miniprep of broth

  • The broth, which contains cells that have been transformed with pSB1C3/TOD genes, was minipreped using the Omega Bio-Tek kit.
  • This would enable us to confirm which cells have the recircularised plasmid and those with the ligated product.

Measurement of DNA concentration

Gel electrophoresis of isolated plasmids

  • The plasmids that were isolated from the miniprep were run on a 1% agarose gel to confirm distinguish between cells that have the pSB1C3/TOD genes from those that have the recircularised pSB1C3 vector.
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