Team:Groningen/Labwork/9 September 2013

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Revision as of 13:32, 9 September 2013 by Claudio (Talk | contribs)

Claudio

S13 and S14 were digested with XbaI and PstI and purified.
pSB1C3-S1-S5 and pSB1C3-S2-S5 were digested with SpeI and PstI.
pSB1C3-S1-S5 and pSB1C3-S2-S5 were ligated to S13 and S14, respectively.
The ligation product was transformed in E. Coli DH5α and plated on LB + Cm.

S3 and S9 were digested with BamHI and PstI.
S16 was digested with EcoRI and BamHI.
S16 was ligated to S3 and S9.
The ligation reaction was run on gel 0.8% to check the presence of product.

Sebas