lab 208

Main purpose

Who was in the lab

Julia, Kristian, Henrike

Procedure

Colony PCR to verify inserts HAO and Nir in pZA21::ara and pZA21::ara tight

Results

Transfomation

Transformation on last friday gave the following results:

• HAO in pZA21::ara (from Biobrick K808000) - 6 colonies (3 orange and small, 3 normal color and very big)
• negative HAO in pZA21::ara (from Biobrick K808000) - no colonies

• HAO in pZA21::ara tight - a lot of colonies, some very big
• negative HAO in pZA21::ara tight - a lot of small colonies

• Nir in pZA21::ara tight - 8 colonies
• negative Nir in pZA21::ara tight - no colonies