From 2013.igem.org

Backbone

To get our developed biobricks into b. subtilis, a backbone was developed. The biobrick compatible backbone developed by the iGEM team of Munich 2012 (Insert exact name) was used as an template but is improved (insert link to part registry) . A hyspank promoter was added to the backbone. The multiple cloning site is used to enter our biobricks into the system. For the use of b. subtilis. the amyE sites are used to cut it