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Team:Groningen/Labwork/13 September 2013
From 2013.igem.org
Claudio
The plates pSB1C3-S16-3-5 and pSB1C3-S16-9-11 showed around thousands of colonies.Colony PCR was performed (NEB Mater Mix) on 4 colonies per plate using VF2 and VR primers (annealing temperature 64°C).
Colony C from pSB1C3-S16-3-5 and colony D from pSB1C3-S16-9-11 were positive candidate and were inoculated in LB + Cm.
Inne
Ran 2 gels with colony pcr samples.Gel 1:
| Ladder | s16-3-5 A | s16-3-5 B | s16-3-5 C | s16-3-5 D | s16-9-11 A | s16-9-11 B | s16-9-11 C | s16-9-11 D | Positive control |
Gel 2
| Ladder | 1 A | 1 B | empty | empty | 2 A | 2 B | 3 A | 3 B | 4 A | 4 B |
| Ladder | 5 A | 5 B | 6 A | 6 B | 7 A | 7 B | 8 A | 8 B | empty | empty |
iGEM 2013 Groningen
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