Team:Groningen/Labwork/14 September 2013

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Claudio

Plates containing BBa_K1085014-S1-S5-S14 and BBa_K1085014-S2-S5-S13 showed around 20 colonies.
Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using F_spank_NheI and HM14 primers (annealing temperature 65°C).
The samples were checked on agarose gel 0.8%.

Plates containing pSB1C3-S1-S5-S13 and pSB1C3-S2-S5-S14 showed around 20 colonies.