Team:Groningen/Labwork/14 September 2013
From 2013.igem.org
Claudio
Plates containing BBa_K1085014-S1-S5-S14 and BBa_K1085014-S2-S5-S13 showed around 20 colonies.Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using F_spank_NheI and HM14 primers (annealing temperature 65°C).
The samples were checked on agarose gel 0.8%.
Plates containing pSB1C3-S1-S5-S13 and pSB1C3-S2-S5-S14 showed around 20 colonies.