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Team:DTU-Denmark/Notebook/11 June 2013
From 2013.igem.org
11 June 2013
Contents |
208 lab
Main purposes today
helloworld-project
- diluting primers with 1.5 ml milli Q water each
- diluting G-block with 20ul milli Q water
Who was in the lab
Procedure
- 7 rxns in duplicates
- mastermix for 15 rxns
| per rxn | master | |
|---|---|---|
| 5x HF buffer | 10ul | 150ul |
| MgCl_2 | 0.5ul | 7.5ul |
| dNTPs | 5ul | 75ul |
| Phusion polymerase | 0.5ul | 7.5ul |
| Milli Q water | 25ul | 375ul |
| master mix total | 615 ul |
Every PCR tube contains
| mastermix | 41ul | |
| FW primer | 3ul | |
| RV primer | 3ul | |
| DNA template | 1ul | |
| PCR tube total | 50ul |
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