June 24
Wet lab
· Inoculation of pBlueScript KS(+) for training
· Autoclave basic materials
· Preparing LB
· LB-Ampicillin plates poured Dry lab
Protocols review

June 25
Wet lab
· Plasmid extraction of pBlueScript KS(+)
· LB-Chloramphenicol plates poured
Dry lab
· Primers design for FABP1, PPARa, GRP78
· Informal meeting

June 26
Wet lab
· Extraction of gDNA from HepG2 Cells for FABP1 and PPARa
· Transformation of pEGFP-N1
· Transformation of BBa_K817002 (pFadBA)

June 27
Wet lab
· Inoculation of pEGFP-N1 for plasmid extraction
· Inoculation of BBa_K817002 (pFadBA) for plasmid extraction

June 28
Wet lab
· Extraction of pEGFP-N1 plasmids by miniprep
· Extraction of BBa_K817002 (pFadBA)
· Restriction of pEGFP-N1 Restriction digestion by Ase1 and bamH1 for PPARa; Ase1 and Xho1 for GRP78, Xba1 and BamH1 for FABP1, Pst1 and Not1; followed by gel check, extraction and purification
· Restriction of c(pFadBA) by EcoR1 and Pst1
· Ran 0.8% gel for pEGFP-N1
· Ran 2% gel for BBa_K817002 (pFadBA)

June 30
Dry lab
Experiment planning and protocols revision for next week work