Transformed iDUET101
Mini prepped transformant and do digestion check
Make glycerol stock out of iDUET101
Extract out Psb1C3 from BBa_J04450 using gel extraction with XbaI and PstI
Extract E. coli BW25113 genome

Ligation of aceA and aceB with pSB1c3
Transformed aceA-pSB1C3 and aceB-pSB1C3
Screen out colonies for aceA-pSB1C3 and aceB-pSB1C3 and do digestion check
aceB-pSB1C3 confirmed and proceed with sequencing
aceB-pSB1C3 partial sequence confirmed

Extracted out more Psb1c3 from BBa_J04450
PCR out aceA with standard prefix and suffix
Digestion of aceA with XbaI and PstI
Ligate with pSB1C3
Screen colonies and do digestion check
aceA-pSB1C3 confirmed and proceed with sequencing
aceA-pSB1C3 partial sequence confirmed
Help Chloe do digestion check for EF1a-pSB1C3

Extraction of EF1a for Biobrick
Extract out more pSB1C3 from BBa_J04450
Digestion of EF1a with EcoRI and PstI
Ligation of EF1a with pSB1c3
Transformed EF1a-pSB1C3 construct
Screen colonies and do digestion check
Weird bands show up, do colony PCR to confirm whether EF1a is ligated
Colony PCR showed that EF1a is there
Colony PCR with VF2 and VR primers didn’t give any result