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Team:UCSF/Project/Attribute
From 2013.igem.org
Throughout the entire summer, each student had specific jobs that contribute to our project. There are moments throughout the summer that the team comes together and work together for a common goal. At the beginning of the summer, everyone came together in the research and brain storming that eventually ended up with the conjugation and synethic circuit project. Every Monday, everyone had to attend a group meeting with their own slides and present a weekly update to both the students and the mentor on their weekly progress. The team worked together in order to set up and execute the Exploratorium project successfully for our human practices. Each student had different roles in providing the content for the wiki page and our coordinators, Veronica Zepeda and Kara Helmke, helped upload the content to the webpage.
- For the Lincoln Project, majority of the recently graduated Lincoln students worked on it with Kendall Kearns and Priyanka Dadlani.
- Priyanka Dadlani was the leader of the modeling for both of our projects. She did most of the majority of the modelling for the projects and worked on the graphs for the project. Priyanka also worked on the human practices by creating the description and help organizing the content on it.
- David Dinh was the leader of submitting the required parts to the iGEM parts registry. He, Verna Huang, Kendall Kearns, and Sherry Teng worked together in creating the parts and building the cassettes necessary for the conjugation project. David was also the leader in all the collaborations project the team had done over the entire summer. He also cloned the pBAD promoter at the beginning of the summer.
- Ian Ergui and Felicity Jika specifically worked on the parts for the synthetic circuit project. They both worked together on inserting the gRNAs for XylE and LacZ into the pCOLA plasmid. At the same time, they also worked on inserting the XylE and LacZ gene in the pCOLA plasmid. Both of them also worked together for the modelling of the Synthetic Circuit project. Ian worked on the primers for the GFP cassette for the conjugation project.
- Derrick Lee worked on the creation of plasmid A for the synthetic circuit by inserting the GFP & RFP and the gRNAs for both of the fluorescent proteins. Later in the summer, Derrick Lee & Eric Wong worked together in the conjugation assay and testing the conjugation rate between the JM109 and S17-1. He also worked on the modelling for the Synthetic Circuit project
- Sherry Teng worked mostly on cloning the dCas9 that was required in both of the project. At the beginning of the summer, Sherry cloned the pTet at the beginning of the summer. She and Kendall Kearns worked on creating the RFP cassette for the conjugation project. She also worked on the modelling for the synthetic circuit.
- Eric Wong worked on the promoter assay for the Synthetic Circuit in order to characterized and figure out which promoters we should use in the circuit. He was the leader of the conjugation assay and worked with Derrick Lee in the assay. He also worked on the modelling for the conjugation project.
- Verna Huang worked with Sherry Teng on the creation of the RFP cassette for the conjugation project. Verna was the leader on the knock in of the cassettes for the conjugation project. She also cloned the pLac promoters for the synthetic circuit project. She and Ji Weiyue worked together in the conjugation assay and testing if conjugation was successful and if the strains was producing the desired fluorescent colors. Verna worked on analyzing the colonies of the conjugation project through microscopy. She also worked on the modeling for the Conjugation project.
- Ji Weiyue (Lily) worked on basic layout of the website and eventually created the final design of our website. Lily worked on the data analysis for both of the projects and helped created the graph for both of the projects. She collected data from the conjugation project through the use of flow cytometry. Lily was the leader on working on changing the promoter’s affinity for the synthetic circuit project.
- Kendall Kearns worked on the GFP cassette for the conjugation project. She and Verna Huang worked together in the conjugation assay and the fluorescent
