Team:Groningen/Labwork/20 September 2013

From 2013.igem.org

Revision as of 13:21, 20 September 2013 by Claudio (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Claudio

The plate containing ligation product BBa_K1085014-BBa_K1085018 showed around a hundreds of colonies.
Colony PCR (NEB Master Mix) was performed on 16 colonies using F-hy_spank-NheI and HM14 primers (annealing temperature 70°C).
The samples were checked on agarose gel 0.8%.

Colony 13 from plate BBa_K1085014-BBa_K1085018 showed to be a positive candidate and was inoculated in LB + Amp.