Template:Kyoto/Notebook/Aug 30

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Contents

Aug 30

Gel Extraction

No Name

Namequantityconcentration[µg/mL]260/280260/230
PT181 atte(2) E+S305.3 2.2 1.66 0.36
PT181 atte(2) X+P217.7 3.9 1.73 0.04
PT181 anti(2) E+S347.7 3.1 1.88 0.31
PT181 anti(2) X+P376.5 5.9 0.49 2.36
PSBIC3 E+S436.6 30.7 1.03 1.28
PSBIC3 X+P457.4 7.9 1.65 0.42
Spinach E+S490.5 3.0 1.87 0.26
tetR aptamer 12-1R E+S451.7 -12.8 5.56 -0.04

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
RBS-lysis2 9264.6 1.97 2.21
RBS-lysis2 12220.0 1.89 2.46
RBS-lysis2 14195.3 1.88 1.53

Colony PCR

No name

Samplebase pair
8/29 Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT(8/20 LB Amp) (1)~(4)2143
8/29 Plux-RBS-GFP-DT(8/27 LB-CP) (1)~(2)1227
8/29 Pbad-larac-RBS-RFP{BBaI13516} (8/21 LB-CP) (1)~(2)2256
8/29 RBS-lysis3-DT-(1)1210
PreDenatureDenatureAnnealingExtensioncycle
 94°C 94°C 55°C 68°C--
5min 30s 30s 2min 30cycle

Ethanol Precipitation

No name

DNA
pT181 atte-(2) E+S
pT181 atte-(2) X+P
pT181 anti-(2) E+S
pT181 anti-(2) X+P
pSB1C3 E+S
pSB1C3 X+P
Spinach E+S
tetR aptamer 12_1R E+S

Restriction Enzyme Digestion

No name

8/30 RBS-lysis2 (9)EcoRISpeI10x bufferB100x BSAMilliQtotal
2 cuts7.5 µL0.5 µL0.5 µL3 µL0.3 µL18.2 µL30 µL
NC0.5 µL0 µL0 µL1 µL0.1 µL8.4 µL10 µL
8/22 pSB1C3-(2)EcoRISpeI10x Buffer100x BSAMilliQtotal
2 cuts8 µL0.5 µL0.5 µL3 µL0.3 µL17.7 µL30 µL
NC1 µL0 µL0 µL1 µL0.1 µL7.9 µL10 µL
8/22 pSB1C3-(2)XbaIPstIBufferDBSAMilliQtotal
2 cuts8 µL0.5 µL0.5 µL3 µL0.3 µL17.7 µL30 µL
NC1 µL0 µL0 µL1 µL0.1 µL7.9 µL10 µL
8/21 tRNA-Spinach (1)EcoRISpeI10x BufferB100x BSAMilliQtotal
2 cuts4 µL0.5 µL0.5 µL3 µL0.3 µL21.9 µL30 µL
NC0.5 µL0 µL0 µL1 µL0.1 µL8.4 µL10 µL
8/21 tetR aptamer 12_1R (1)EcoRISpeI10xBufferB100xBSAMilliQtotal
2 cuts3 µL0.5 µL0.5 µL3 µL0.3 µL22.7 µL30 µL
NC0.4 µL0 µL0 µL1 µL0.1 µL8.4 µL10 µL
8/21 pT181 attenuator-(2)EcoRISpeIXbaIPstI10x BufferB10x BufferD100x BSAMilliQtotal
2 cuts4 µL0.5 µL0.5 µL0 µL0 µL3 µL0 µL0.3 µL21.7 µL30 µL
NC0.5 µL0 µL0 µL0 µL0 µL1 µL0 µL0.1 µL8.4 µL10 µL
2 cuts4 µL0 µL0 µL0.5 µL0.5 µL0 µL3 µL0.3 µL21.7 µL30 µL
NC0.5 µL0 µL0 µL0 µL0 µL0 µL1 µL0.1 µL8.4 µL10 µL
8/21 pT181 antisense-(2)EcoRISpeIXbaIPstI10x BufferB10x BufferD100x BSAMilliQtotal
2 cuts9 µL0.5 µL0.5 µL0 µL0 µL3 µL0 µL0.3 µL16.7 µL30 µL
NC1 µL0 µL0 µL0 µL0 µL1 µL0 µL0.1 µL7.9 µL10 µL
2 cuts9 µL0 µL0 µL0.5 µL0.5 µL0 µL3 µL0.3 µL16.7 µL30 µL
NC1 µL0 µL0 µL0 µL0 µL0 µL1 µL0.1 µL7.9 µL10 µL