Team:SCUT

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Abstract

E.cerevisiae is a sophisticated signal transport system between E.coli and S.cerevisiae. Producer, the E.coli, is assigned to distribute a special volatile—butanedione periodically with a stable oscillation circuit, which defines the meaning of the signal. On the other side, Sniffer,the yeast, transplanted with a nose from nematode, can respond to the signal immediately. We hope this can realize the communication between prokaryotes and eukaryotes for the further research on symbiosis.

Oscillating odorant contains two parts: the Producer and the Oscillator. We choose diacetyl as our desire odorant which widely used as a food additive to emit buttery flavor. In order to enhance the production, we construct the E.coli as the producer . Additionally, we add the oscillation system on the basis of odorant producing system, together, distributing a special volatile—butanedione periodically.

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