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Team:Georgia State/Notebook/july
From 2013.igem.org
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We continued performing digestions, ligations, and transformations without being able to confirm insertion of the pGAPZαC MCS linker into pGAPZαC.
Week 9: 7/8 -7/12
We continued performing digestions, ligations, and transformations without being able to confirm insertion of the pGAPZαC MCS linker into pGAPZαC.
We also performed a midiprep on pGAPZαA + MCS linker + RFP, made and filtered sterilized 1L 10% glycerol, made LB agar for chloramphenicol plates, and prepared electrocompetent E. coli.
Week 10: 7/15 – 7/20
We had problems with arching during Electrotransporation of E. coli so we switched to transforming cells chemically.
Week 11: 7/23 – 7/27
We realized that the RFP that we had inserted in pGAPZαA + pGAPZαA MCS linker last year was not the optimal version of RFP to be inserted in yeast so we transformed E. coli with the what we thought was the more optimal RFP, but we picked the RFP from the wrong kit plate.
Week 12: 7/29 – 8/2
We transformed from Kit Plate 3 N12 RFP into E. coli and performed minipreps on an overnight culture from one of the transformed colonies.
Group C
Week 8: 7/1 – 7/5We continued performing digestions, ligations, and transformations without being able to confirm insertion of the pGAPZαC MCS linker into pGAPZαC.
Week 9: 7/8 -7/12
We continued performing digestions, ligations, and transformations without being able to confirm insertion of the pGAPZαC MCS linker into pGAPZαC.
We also performed a midiprep on pGAPZαA + MCS linker + RFP, made and filtered sterilized 1L 10% glycerol, made LB agar for chloramphenicol plates, and prepared electrocompetent E. coli.
Week 10: 7/15 – 7/20
We had problems with arching during Electrotransporation of E. coli so we switched to transforming cells chemically.
Week 11: 7/23 – 7/27
We realized that the RFP that we had inserted in pGAPZαA + pGAPZαA MCS linker last year was not the optimal version of RFP to be inserted in yeast so we transformed E. coli with the what we thought was the more optimal RFP, but we picked the RFP from the wrong kit plate.
Week 12: 7/29 – 8/2
We transformed from Kit Plate 3 N12 RFP into E. coli and performed minipreps on an overnight culture from one of the transformed colonies.
