From 2013.igem.org
Aug 17
Colony PCR
Nakamoto
| Sample | base pair
|
| 8/15 BBa_K112806(lysis2) -(1) | 828
|
| negative control | --
|
| PreDenature | Denature | Annealing | Extension | cycle
|
| 94°C | 94°C | 55°C | 68°C | --
|
| 5min | 30s | 30s | 1min | 30cycles
|
| Sample | base pair
|
| 8/10 BBa_Q0400 -(1) | 3995
|
| negative control | --
|
| PreDenature | Denature | Annealing | Extension | cycle
|
| 94°C | 94°C | 55°C | 68°C | --
|
| 5min | 30s | 30s | 30s | 30cycles
|
File:Igku Aug19electrophoresis2.png
Colony PCR
Nakamoto
| Sample | base pair
|
| 8/15 BBa_K112806(lysis2) -(1) | 828
|
| negative control | --
|
| PreDenature | Denature | Annealing | Extension | cycle
|
| 94°C | 94°C | 55°C | 68°C | --
|
| 5min | 30s | 30s | 1min | 30cycles
|
| Sample | base pair
|
| 8/10 BBa_Q0400 -(1) | 3995
|
| negative control | --
|
| PreDenature | Denature | Annealing | Extension | cycle
|
| 94°C | 94°C | 55°C | 68°C | --
|
| 5min | 30s | 30s | 30s | 30cycles
|
File:Igku Aug19electrophoresis2.png
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