Template:Kyoto/Notebook/Sep 12

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Contents

1 Sep 12

Sep 12

Miniprep

Nakamoto

DNA	concentration[µg/mL]	260/280	260/230
Pcon-pT181atte-DT	293.6	1.88	1.84
Pcon-apt12-1R-DT	303.8	1.75	1.64
Fusion1atte(pSB1c3)	172.0	1.96	2.31
RBS-lisis1-DT	235.1	1.59	1.60
RBS-lisis2-DT	387.7	1.91	2.01
RBS-lisis3-DT	387.9	1.91	2.09
Plux	166.7	1.98	1.63

Electrophoresis

Nakamoto

Lane	Sample
1	100bp ladder
2	9/11 P-RBS-lisis1-DT(Colony PCR prodution)

File:Igku xxxxxx.xxx

Liquid Culture

Nakamoto

Sample	medium
9/10 Pcon-apt12-1R-DT1	Plusgrow medium(+Amp)
9/10 DT-Pcon-pT181atte1	Plusgrow medium(+CP)
9/10 Fusion1-atte(pSB1c3)	Plusgrow medium(+CP)

v>

Electrophoresis

Tatsui

1

Lane	Sample
1	1kb ladder
2	pSB4K5(EcoR1&Spe1)
3	pSB4K5 NC(EcoR1&Spe1)
4	RBS-lacz-DT(EcoR1&Spe1)
5	EcoR1&Spe1 NC( EcoR1&Spe1)
6	Pcon-RBS-lacz-DT( EcoR1&Spe1)
7	Pcon-RBS-lacz-DT NC( EcoR1&Spe1)
8	1kb ladder

File:Igku xxxxxx.xxx

2
}

File:Igku xxxxxx.xxx

Lane	Sample
1	1kb ladder
2	Ptet-pT181anti(Spe1&Pst1)
3	Ptet-pT181anti NC(Spe1&Pst1)
4	RBS-lysis2-DT(Xba1&Pst1)
5	EcoR1&Spe1 NC( EcoR1&Spe1)
6	--
7	--
8	1kb ladder

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