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Template:Kyoto/Notebook/Sep 12
From 2013.igem.org
Contents |
Sep 12
Miniprep
| DNA | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| Pcon-pT181atte-DT | 293.6 | 1.88 | 1.84 |
| Pcon-apt12-1R-DT | 303.8 | 1.75 | 1.64 |
| Fusion1atte(pSB1c3) | 172.0 | 1.96 | 2.31 |
| RBS-lisis1-DT | 235.1 | 1.59 | 1.60 |
| RBS-lisis2-DT | 387.7 | 1.91 | 2.01 |
| RBS-lisis3-DT | 387.9 | 1.91 | 2.09 |
| Plux | 166.7 | 1.98 | 1.63 |
Electrophoresis
| Lane | Sample |
|---|---|
| 1 | 100bp ladder |
| 2 | 9/11 P-RBS-lisis1-DT(Colony PCR prodution) |
Liquid Culture
| Sample | medium |
|---|---|
| 9/10 Pcon-apt12-1R-DT1 | Plusgrow medium(+Amp) |
| 9/10 DT-Pcon-pT181atte1 | Plusgrow medium(+CP) |
| 9/10 Fusion1-atte(pSB1c3) | Plusgrow medium(+CP) |
Electrophoresis
1
| Lane | Sample |
|---|---|
| 1 | 1kb ladder |
| 2 | pSB4K5(EcoR1&Spe1) |
| 3 | pSB4K5 NC(EcoR1&Spe1) |
| 4 | RBS-lacz-DT(EcoR1&Spe1) |
| 5 | EcoR1&Spe1 NC( EcoR1&Spe1) |
| 6 | Pcon-RBS-lacz-DT( EcoR1&Spe1) |
| 7 | Pcon-RBS-lacz-DT NC( EcoR1&Spe1) |
| 8 | 1kb ladder |
2
| Lane | Sample |
|---|---|
| 1 | 1kb ladder |
| 2 | Ptet-pT181anti(Spe1&Pst1) |
| 3 | Ptet-pT181anti NC(Spe1&Pst1) |
| 4 | RBS-lysis2-DT(Xba1&Pst1) |
| 5 | EcoR1&Spe1 NC( EcoR1&Spe1) |
| 6 | -- |
| 7 | -- |
| 8 | 1kb ladder |
Gel Extraction
| Lane | DNA | Enzyme | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 1kb ladder | -- | |||||||||||||
| 2 | RBS-lacZ-DT | EcoR1&Spe1 | |||||||||||||
| 3 | RBS-lacZ-DT | EcoR1&Spe1 | |||||||||||||
| 4 | RBS-lacZ-DT | EcoR1&Spe1 | |||||||||||||
| 5 | -- | -- | |||||||||||||
| 6 | Pcon-RBS-lacZ-DT | EcoR1&Spe1 | |||||||||||||
| 7 | Pcon-RBS-lacZ-DT | EcoR1&Spe1 | |||||||||||||
| 8 | Pcon-RBS-lacZ-DT | EcoR1&Spe1 | |||||||||||||
| 9 | 1kb ladder | -- |
}
File:Igku Aug19electrophoresis3
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