"
Template:Kyoto/Notebook/Sep 17
From 2013.igem.org
Sep 17
Liquid Culture
| Sample | medium |
|---|---|
| Spi-DT | LB medium(+CP) |
| Pcon-Spi-DT,Pcon-pT181-anti-spi-DT | LB medium(+Amp) |
PCR
| Pcon-tet ap-DT(VF) | BigDye | 5x buffer | primer(VF) | MilliQ | total |
|---|---|---|---|---|---|
| 1.3 | 0.5 | 1.75 | 0.5 | 0.45 | 10.5 |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 96°C | 96°C | 50°C | 60°C | -- |
| 2m | 10s | 5s | 4m | 30cycles |
| Pcon-tet ap-DT(VR) | BigDye | 5x buffer | primer(VR) | MilliQ | total |
|---|---|---|---|---|---|
| 1.3 | 0.5 | 1.75 | 0.5 | 0.45 | 10.5 |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 96°C | 96°C | 50°C | 60°C | -- |
| 2m | 10s | 5s | 4m | 30cycles |
