From 2013.igem.org
Sep 23
じっけんしゃ
| Lane | DNA | Enzyme
|
| 1 | 1kbp ladder |
|
| 3-4 | Pcon-RBS-tetR-PT |
|
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | concentration[µg/mL] | 260/280 | 260/230
|
| Pcon-PT181 anti E+S | 46.7 | 1.06 | 0.93
|
| Pcon-RBS-tetR-DT E+X | 119.3 | 0.99 |
|
| RBS-8FP-DT E+X | 87.7 | 1.00 | 0.98
|
| ap42_1R-DT E+X | 68.8 | 1.32 | 1.03
|
| Pcon-RBS-tetR-DT 1-2 A | 203.0 | 1.66 | 1.79
|
| Pcon-RBS-tetR-DT 3-2 A | 67.7 | 2.64 | 1.28
|
| Pcon-RBS-GFP-DT 1-S A | 185.6 | 1.88 | 2.04
|
| Pcon-RBS-GFP-DT 2-S A | 298.6 | 1.68 | 1.55
|
| Pcon-tetR-DT E-2 A | 202.4 | 1.57 | 1.66
|
PCR
No name
| genome DNA | KOD plus | 10x buffer | dNTP | MgSO4 | SasA_fwd primer | SasA_rev primer | MilliQ | total
|
| 9/8 Pcon-pT181 anti | 0.5 | 2.5 | 2.5 | 1.5 | 0.75 | 0.75 | 15.5 | 25
|
| 8/20 Ptet(1) | 0.5 | 2.5 | 2.5 | 1.5 | 0.75 | 0.75 | 15.5 | 25
|
| 9/16 Plac 2 | 0.5 | 2.5 | 2.5 | 1.5 | 0.75 | 0.75 | 15.5 | 25
|
| PreDenature | Denature | Annealing | Extension | cycle
|
| 94 °C | 98 °C | 57 °C | 68 °C | --
|
| 2min | 10sec | 30sec | 20sec | 30cycles
|
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