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Template:Kyoto/Notebook/Sep 4
From 2013.igem.org
Contents |
Sep 4
Gel Extraction
1
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | -- |
| 2 | Pcon | SpeI&PstI |
| 3 | Pcon | SpeI&PstI |
| 4 | -- | -- |
| 5 | Plac | SpeI&PstI |
| 6 | Plac | SpeI&PstI |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| Pcon | -- | 13.2 | 1.99 | -1.64 |
| Plac | -- | 15.3 | 2.07 | -o.39 |
2
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | -- |
| 2 | Ptet | SpeI&PstI |
| 3 | Ptet | SpeI&PstI |
| 4 | -- | -- |
| 5 | RBS-tetR-DT | XbaI&PstI |
| 6 | RBS-tetR-DT | XbaI&PstI |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| Ptet | -- | 30.1 | 1.97 | -0.97 |
| RBS-tetR-DT | -- | 3.0 | 2.44 | 0.88 |
Gel Extraction
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | -- |
| 2 | spinach-DT | XbaII&PstI |
| 3 | spinach-DT NC | XbaII&PstI |
| 4 | RBS-lacZα-DT | XbaI&PstI |
| 5 | RBS-lacZα-DT NC | XbaI&PstI |
| 6 | 100bp ladder | -- |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| spinach-DT | -- | 2.1 | 2.10 | -0.05 |
| RBS-lacZα-DT | -- | 3.3 | 2.59 | -0.07 |
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Master Plate
| Number | Use LB plate (+Amp) |
|---|---|
| 4 | 8/9 BBa_J23100-3 |
| 5 | 8/9 BBa_J23100-4 |
| 6 | 8/9 BBa_J23100-5 |
Master Plate
| Number | Use LB plate |
|---|---|
| 1 | JM109-1 |
| 2 | JM109-2 |
| 3 | JM109-3 |
| 4 | JM109-4 |
Liquid Culture
| Sample | medium |
|---|---|
| 8/9 BBa_J23100-3 | Plusgrow medium(+Amp) |
Liquid Culture
| Sample | medium |
|---|---|
| JM109-1(Master plate) | LB medium |
Colony PCR
| Sample | base pair |
|---|---|
| 8/9 BBa_J23100 3 | -- |
| 8/9 BBa_J23100 4 | -- |
| 8/9 BBa_J23100 5 | -- |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 94°C | 94°C | 55°C | 68°C | -- |
| 5min | 30s | 30s | 30s | 30cycles |
