Template:Kyoto/Notebook/Aug 23

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Aug 23

No name

Lane	Sample	Enzyme1	Enzyme2
1	pT181 antisense	EcoRI	SpeI
2	pT181 antisense	EcoRI	--
3	pT181 antisense	--	SpeI
4	pT181 antisense	--	--
5	pT181 antisense	XbaI	PstI
6	pT181 antisense	XbaI	--
7	pT181 antisense	--	PstI
8	pT181 antisense	--	--
9	100bp ladder	--	--
10	Fusion1 attenuator	EcoRI	SpeI
11	Fusion1 attenuator	EcoRI	--
12	Fusion1 attenuator	--	SpeI
13	Fusion1 attenuator	--	--
14	Fusion1 attenuator	XbaI	PstI
15	Fusion1 attenuator	XbaI	--
16	Fusion1 attenuator	--	PstI
17	Fusion1 attenuator	--	--

Lane	Sample	Enzyme1	Enzyme2
1	Fusion3m2 attenuator	EcoRI	SpeI
2	Fusion3m2 attenuator	EcoRI	--
3	Fusion3m2 attenuator	--	SpeI
4	Fusion3m2 attenuator	--	--
5	Fusion3m2 attenuator	XbaI	PstI
6	Fusion3m2 attenuator	XbaI	--
7	Fusion3m2 attenuator	--	PstI
8	Fusion3m2 attenuator	--	--
9	100bp ladder	--	--
10	tetR aptamer 12_1M	EcoRI	SpeI
11	tetR aptamer 12_1M	EcoRI	--
12	tetR aptamer 12_1M	--	SpeI
13	tetR aptamer 12_1M	--	--
14	tetR aptamer 12_P	EcoRI	SpeI
15	tetR aptamer 12_P	EcoRI	--
16	tetR aptamer 12_P	--	SpeI
17	tetR aptamer 12_P	--	--

Kojima and Honda

Lane	Sample	Enzyme1	Enzyme2
1	Plac	SpeI	PstI
2	Plac	SpeI	--
3	Plac	--	PstI
4	Plac	--	--
5	Spinach	EcoRI	SpeI
6	Spinach	EcoRI	--
7	Spinach	--	SpeI
8	Spinach	--	--
9	100bp ladder	--	--
10	J23100	SpeI	PstI
11	J23100	SpeI	--
12	J23100	--	PstI
13	J23100	--	--
14	tetR aptamer 12_1R	EcoRI	SpeI
15	tetR aptamer 12_1R	EcoRI	--
16	tetR aptamer 12_1R	--	SpeI
17	tetR aptamer 12_1R	--	--

Nakamoto and Kojima

	Fusion6 antisense (1)	EcoRI	SpeI	buffer B	BSA	MilliQ	total
2 cuts	3	0.5	0.5	3	0.3	22.7	30
1 cut	0.6	0.1	0	1	0.1	8.2	10
1 cut	0.6	0	0.1	1	0.1	8.2	10
NC	0.6	0	0	1	0.1	8.3	10

	Fusion6 antisense (1)	XbaI	PstI	buffer D	BSA	MilliQ	total
2 cuts	3	0.5	0.5	3	0.3	22.7	30
1 cut	0.6	0.1	0	1	0.1	8.2	10
1 cut	0.6	0	0.1	1	0.1	8.2	10
NC	0.6	0	0	1	0.1	8.3	10

	Fusion1 antisense (1)	EcoRI	SpeI	buffer B	BSA	MilliQ	total
2 cuts	3.3	0.5	0.5	3	0.3	22.4	30
1 cut	0.7	0.1	0	1	0.1	8.1	10
1 cut	0.7	0	0.1	1	0.1	8.1	10
NC	0.7	0	0	1	0.1	8.2	10

	pT181 antisense (1)	EcoRI	SpeI	buffer B	BSA	MilliQ	total
2 cuts	5.6	0.5	0.5	3	0.3	20.1	30
1 cut	1.1	0.1	0	1	0.1	7.7	10
1 cut	1.1	0	0.1	1	0.1	7.7	10
NC	1.1	0	0	1	0.1	7.8	10

	pT181 antisense (1)	XbaI	PstI	buffer D	BSA	MilliQ	total
2 cuts	5.6	0.5	0.5	3	0.3	20.1	30
1 cut	1.1	0.1	0	1	0.1	7.7	10
1 cut	1.1	0	0.1	1	0.1	7.7	10
NC	1.1	0	0	1	0.1	7.8	10

No name

Lane	Sample	Enzyme1	Enzyme2
1	100bp	--	--
2	pT181 attenuator	EcoRI	SpeI
3	pT181 attenuator	XbaI	PstI
4	Fusion3m2 attenuator	EcoRI	SpeI
5	Fusion1 attenuator	XbaI	PstI
6	Fusion1 attenuator	EcoRI	SpeI
7	Fusion3m2 attenuator	XbaI	PstI
8	pT181 antisense	EcoRI	SpeI
9	pT181 antisense	XbaI	PstI
10	Fusion1 antisense	EcoRI	SpeI
11	Fusion1 antisense	XbaI	PstI
12	Fusion6 antisense	EcoRI	SpeI
13	Fusion6 antisense	XbaI	PstI
14	tetR aptamer 12_1M	EcoRI	SpeI
15	tetR aptamer 12_P	EcoRI	SpeI
16	tetR aptamer 12_1R	EcoRI	SpeI
17	100bp	--	--

Lane	Sample	Enzyme1	Enzyme2
1	100bp	--	--
2	Spinach	EcoRI	SpeI
3	J23100	SpeI	PstI
4	Plac	SpeI	PstI
5	100bp	--	--
6	J23100	SpeI	PstI

Lane	Sample	Enzyme1	Enzyme2
1	100bp	--	--
2	Fusion6 antisense	EcoRI	SpeI
3	Fusion6 antisense	EcoRI	--
4	Fusion6 antisense	--	SpeI
5	Fusion6 antisense	--	--
6	pT181 antisense	XbaI	PstI
8	100bp	--	--
9	100bp	--	--
10	Fusion6 antisense	XbaI	PstI
11	Fusion6 antisense	XbaI	--
12	Fusion6 antisense	--	PstI
13	Fusion6 antisense	--	--
14	pT181 antisense	XbaI	--
16	100bp	--	--

Lane	Sample	Enzyme1	Enzyme2
1	100bp	--	--
2	Fusion1 antisense	EcoRI	SpeI
3	Fusion1 antisense	EcoRI	--
4	Fusion1 antisense	--	SpeI
5	Fusion1 antisense	--	--
6	pT181 antisense	--	PstI
8	100bp	--	--
9	100bp	--	--
10	pT181 antisense	EcoRI	SpeI
11	pT181 antisense	EcoRI	--
12	pT181 antisense	--	SpeI
13	pT181 antisense	--	--
14	pT181 antisense	--	--
16	100bp	--	--

Nakamoto, Ashida, Kojima, Okazaki & Matsumoto

Ashida