Team:UChicago/Protocols

Notebook > Protocols

Protocols page

General Lab Best Practices

Labeling

Label tubes at all time before storage. Label clearly with:

1. Contents in the tube
2. Date
3. Your name/Initials
4. Number

Notes on Sterile Techniques

• Turn the flame on before opening anything sterile.

• Aliquot everything you use to prevent contamination. Before pouring aliquots, flame the bottle rim quickly to keep sterile. Make new aliquots in new tubes with stock when running low.

• Unscrew but do not remove all caps to make it easier to work with in the moment.

• Flame the bottle rim quickly and then aliquot by pouring into 50ml conical tube. Never stick anything in LB bottle, always pour.

• Flame bottle rim again and close LB bottle.

• Take what you need from 50ml tube, keep sterile so it can be used repeatedly.

Lab Notebook Best Practices (read: do these best practices)

Steps to 3A Assembly & Labeling Guidelines

Resuspending DNA from iGEM Kit Plates

Recipes

Making Agar Plates

Pouring the Plates

Making LB (500mL)

Agar Stab Protocols

Ligation Protocol

Transformation Procedures

Making Overnight Cultures

Miniprep Protocols

Making an agarose gel for gel purification

Enzymatic digestion

Gel extraction with the QIAGEN kit