Labwork overview

Bba_K1155000

07/01/13

• Digestion of PSB1C3 plasmid and PCR products : Pfnr by EcoRI/ PstI
• Ligation of PSB1C3 and Pfnr

07/02/13

• Tranformation of Bba_K1155000 in DH5α

07/03/13

• Colony PCR of Bba_K1155000 to check good insertion of Pfnr in PSB1C3
• New culture of Bba_K1155000

07/04/13

• Estimation of Pfnr Colony PCR size fragments thanks to Gene manager
• Electrophoresis of Colony PCR products
• Stock of Bba_K1155000
• Culture of Bba_K1155000
• Extraction of Bba_K1155000 from DH5α
• Digestion of Bba_K1155000 by NotI, MluI, HpaI to check good insertion of Pfnr in PSB1C3 again

07/05/13

• Electrophoresis of the digestion of Bba_K1155000 by NotI, HpaI, MluI
• Extraction of Bba_K1155000 from DH5α

Bba_K1155001

Bba_K1155002

Bba_K1155003

07/02/13

• Tranformation of Bba_K592009 in DH5α

07/03/13

• New culture of Bba_K592009

07/05/13

• Extraction of Bba_K592009 from DH5α

07/08/13

• Digestion of Bba_K592009 by NotI, XhoI, EcoRI, EcoRI/PstI
• Electrophoresis of the digestion of Bba_K592009 by NotI, XhoI, EcoRI, EcoRI/PstI'

Bba_K1155004

Bba_K1155005

Bba_K1155006

Bba_K1155007

07/02/13

• Tranformation of Bba_I732017 in DH5α

07/03/13

• New culture of Bba_I732017

07/05/13

• Extraction of Bba_I732017 from DH5α

07/08/13

• Digestion of Bba_I732017 by NotI, XhoI, EcoRI, EcoRI/PstI
• Electrophoresis of the digestion of Bba_I732017 by NotI, XhoI, EcoRI, EcoRI/PstI'

Bba_K1155008

Bba_K1155009

07/01/13

• Design of oligos for amplification of BphR2 gene of Pseudomonas pseudoalcaligenes, Pseudomonas oleovorans