Team:Penn/Notebook

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Revision as of 03:06, 27 September 2013 by Bkaptur (Talk | contribs)
  1. Learned how to make competent cells, growing up two strains for tomorrow
  2. Transformed 8 plasmids
  3. Determined EL222 fusion is risky but still going ahead with it
  4. Linkers are totally setlled
  5. Found zinc finger plasmid and updated target sequence
  6. Learned how to make tetr- mcherry fusion
  7. Settled on 5 promoters
</li>
  • 5-Jun
    1. Learned how to make competent cells, testing them and then making more tomorrow
    2. Transformed 8 plasmids again
    3. Made primers to clone the TET-GFP reporter system, the mCherry promoter strength system, ready to order
    4. Made ultramers for variable promoter blocks (and no target neg controls) – ready to order
    5. Spilled a lot of iced tea outside, bummer
    6. Started primers for dna binding machines
    7. Got a handle on cas9 fusions (pun intended).
    8. Put awesome pics in dropbox
    </li>
  • 6-Jun
    1. Clean up dropbox
    2. Update budget sheet with addgene and cell center orders
    3. Finish primers for fusion
    4. Set up plate reader for GFP and mCherry assays
    5. run minipreps on pdawn, pdawn-mcherry, pet26b
    6. Grow up mCherry stock
    7. Wrote Penn iGEM on our plasmid
    </li>
  • 7-Jun
    1. Transform
      1. C0012 –amp/chlor (do both)
      2. M11307 – amp/chlor (do both)
      3. I13458 – amp/chlor (do both)
      4. R0010 – amp/chlor (do both)
      5. R0051 – amp
      6. K206000 –chlor
    2. Start the LIMS and file all the strains and DNA we have made/ ordered
    </li>
  • 8-Jun</li>
    1. Miniprep Addgene stuff + transformations that worked
    2. Growing up low copy plasmids in 40mLs
  • Mini-prepped</li>
    1. I9002
    2. I13458
    3. C0051
    4. Pdawn-mcherry
    5. Pdawn
    6. Dhsa mcherry
    7. Pdawn dhsa
    8. Psb1a3
    9. JM mcherry
    10. Pet26b
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