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Team:UC-Santa Cruz/Safety
From 2013.igem.org
1) Please describe the chassis organism(s) you will be using for this project. If you will be using more than one chassis organism, provide information on each of them:
- Species: E. coli
- Strain: K12 derived Life Technologies TOP10 strain
- Risk Group: 1
- Risk Group Source Link: http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli
- Disease risk to humans? If so, which disease?: "E. coli K-12 is not considered a human or animal pathogen nor is it toxicogenic. Any concerns for E. coli K-12 in terms of health considerations are mitigated by its poor ability to colonize the colon and establish infections (http://epa.gov/oppt/biotech/pubs/fra/fra004.htm).
- Species: Caulobacter crescentus
- Strain: CB15 and CB15N
- Risk Group: 1
- Risk Group Source Link: http://www.baua.de/en/Topics-from-A-to-Z/Biological-Agents/TRBA/pdf/TRBA-466.pdf?__blob=publicationFile&v=3
- Disease risk to humans? If so, which disease?: Ubiquitous presence in environmental water supplies including human drinking water and swimming pools. Single case reported of Caulobacter acting as a pathogen in a human.
2) Highest Risk Group Listed: 1
See pg 52 of Caulobacter PDF document above for risk group for Caulobacter crescentus.
3) List and describe all new or modified coding regions you will be using in your project. (If you use parts from the 2013 iGEM Distribution without modifying them, you do not need to list those parts.)
- Part name: BBa_K559000
- Where did you get the physical DNA for this part (which lab, synthesis company, etc): Provided by IGEM
- What species does this part originally come from?: Halobacterium
- What is the Risk Group of the species?: 1
- What is the function of this part, in its parent species?: Rhodopsin pump for establishing a H ion gradient across the cell membrane.
- Part name: DivJ
- Where did you get the physical DNA for this part (which lab, synthesis company, etc): Cloned from Caulobacter cerscentus genomic DNA.
- What species does this part originally come from?: Caulobacter crescentus
- What is the Risk Group of the species?: 1
- What is the function of this part, in its parent species?: Protein tag for targeting a protein to one pole of Caulobacter crescentus.
- Part name: TipF
- Where did you get the physical DNA for this part (which lab, synthesis company, etc): Cloned from Caulobacter cerscentus genomic DNA.
- What species does this part originally come from?: Caulobacter crescentus
- What is the Risk Group of the species?: 1
- What is the function of this part, in its parent species?: Protein tag for targeting a protein to one pole of Caulobacter crescentus.
- Part name: StpX
- Where did you get the physical DNA for this part (which lab, synthesis company, etc): Cloned from Caulobacter cerscentus genomic DNA.
- What species does this part originally come from?: Caulobacter crescentus
- What is the Risk Group of the species?: 1
- What is the function of this part, in its parent species?: Protein tag for targeting a protein to one pole of Caulobacter crescentus.
- Part name: PflI
- Where did you get the physical DNA for this part (which lab, synthesis company, etc): Cloned from Caulobacter cerscentus genomic DNA.
- What species does this part originally come from?: Caulobacter crescentus
- What is the Risk Group of the species?: 1
- What is the function of this part, in its parent species?: Protein tag for targeting a protein to one pole of Caulobacter crescentus.
- Part name: KR2 Na Pumping Rhodopsin
- Where did you get the physical DNA for this part (which lab, synthesis company, etc): Synthesized, using G blocks from IDT
- What species does this part originally come from?: Marine Flavobacterium Krokinobacter eikastus
- What is the Risk Group of the species?: Unlisted, likely 1 based on wide environmental distribution and lack of pathogenicity for marine flavobacterium
- What is the function of this part, in its parent species?: Function is speculated as being for establishing a Na gradient across the cell membrane to drive specific nutrient cotransporters.
