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Template:Kyoto/Notebook/Aug 16
From 2013.igem.org
Contents |
Aug 16
Colony PCR
| Sample | base pair |
|---|---|
| 8/15 BBa_I13504(RBS-GFP-DT)(1) | 1174 |
| 8/15 BBa_I13504(RBS-GFP-DT)(2) | 1174 |
| 8/15 BBa_I0462(RBS-luxR-DT)(1) | 1174 |
| 8/15 BBa_I0462(RBS-luxR-DT)(2) | 1174 |
| 8/15 BBa_K748002(lysis3) | 1053 |
| negative control | -- |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 94°C | 94°C | 55°C | 68°C | -- |
| 5min | 30s | 30s | 1min | 30cycles |
| Sample | base pair |
|---|---|
| 8/15 BBa_R0061(Plux) | 344 |
| 8/15 BBa_K117000(lysis1)(1) | 456 |
| 8/15 BBa_K117000(lysis1)(2) | 456 |
| 8/15 BBa_B0015(DT) | 443 |
| 8/15 BBa_I732020(RBS-lacZα-DT) | 703 |
| negative control | -- |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 94°C | 94°C | 55°C | 68°C | -- |
| 5min | 30s | 30s | 40s | 30cycles |
Electrophoresis
| Lane | Sample |
|---|---|
| 1 | 100bp ladder |
| 2 | BBa_I13504(RBS-GFP-DT) -(1) |
| 3 | BBa_I13504(RBS-GFP-DT) -(2) |
| 4 | BBa_I0462(RBS-luxR-DT) -(1) |
| 5 | BBa_I0462(RBS-luxR-DT) -(2) |
| 6 | BBa_K748002(lysis3) |
| 7 | NC |
| 8 | 100bp ladder |
| Lane | Sample |
|---|---|
| 1 | 100bp ladder |
| 2 | BBa_R0061(Plux) |
| 3 | BBa_K117000(lysis1) -(1) |
| 4 | BBa_K117000(lysis1) -(1) |
| 5 | BBa_B0015(DT) |
| 6 | BBa_I732020(RBS-lacZα-DT) |
| 7 | NC |
| 8 | 100bp ladder |
Master Plate
| Number | Use LB plate (+CP) |
|---|---|
| 1 | BBa_I13504(RBS-GFP-DT)(1) |
| 2 | BBa_I13504(RBS-GFP-DT)(2) |
| 3 | BBa_K748002(lysis3) |
| 4 | BBa_R0061(Plux) |
| 5 | BBa_K117000(lysis1)(1) |
| 6 | BBa_K117000(lysis1)(2) |
| 7 | BBa_B0015(DT) |
| 8 | BBa_I732020(RBS-lacZα-DT) |
| Number | Use LB plate (+Amp) |
|---|---|
| 1 | BBa_I0462(RBS-luxR-DT)(1) |
| 2 | BBa_I0462(RBS-luxR-DT)(2) |
Transformation
| Name | Sample | Competent Cells | Total | Plate |
|---|---|---|---|---|
| pBR322 | 1µL | 100µL(XL-10gold) | 101µL | Amp |
| pBR322 | 1µL | 50µL(takara) | 51µL | Amp |
| Pbad/araC | 2µL | 20µL | 22µL | K |
| RBS-luxI-DT | 2µL | 20µL | 22µL | K |
Liquid Culture
| Sample | medium |
|---|---|
| 8/15 BBa_I13504(RBS-GFP-DT))(1) | Plusgrow medium (+CP) |
| 8/15 BBa_I13504(RBS-GFP-DT)(2) | Plusgrow medium (+CP) |
| 8/15 BBa_I0462(RBS-luxR-DT)(2) | Plusgrow medium (+Amp) |
| 8/15 BBa_K748002(lysis3) | Plusgrow medium (+CP) |
| 8/15 BBa_R0061(Plux) | Plusgrow medium (+CP) |
| 8/15 BBa_K117000(lysis1)(1) | Plusgrow medium (+CP) |
| 8/15 BBa_K117000(lysis1)(2) | Plusgrow medium (+CP) |
| 8/15 BBa_B0015(DT) | Plusgrow medium (+CP) |
| 8/15 BBa_I732020(RBS-lacZα-DT) | Plusgrow medium (+CP) |
