Oscillating odorant contains two parts: the Producer and the Oscillator. We choose diacetyl as our desire odorant which widely used as a food additive to emit buttery flavor. In order to enhance the production, we construct the E.coli as the producer . Additionally, we add the oscillation system on the basis of odorant producing system, together, distributing a special volatile—butanedione periodically.
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Receptor aims to fabricate biomimetic nose and mouth for S.cerevisiae to achieve the communication between eukaryotes and prokaryotes. We select a G-protein coupled receptor (GPCR), odr-10, as our nose to detect diacetyl generated by E.coli. In addition, we use aiiA gene as our mouth, which would translate into aiiA enzyme—an inhibitor to the production of diacetyl secreted from S.cerevisiae with the help of signal peptide.
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