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Team:Groningen/Labwork/27 September 2013
From 2013.igem.org
Claudio
Starch assay was performed on the 6 colonies which were inoculated yesterday.
Colony PCR was also performed on the same 6 colonies. The pair of primers used was F-hy_spank-NheI and HM14 (annealing temperature 70°C).
The samples were checked on agarose gel 0.8%.
The samples showed to be all positive candidates (expected product size ~2400bps).
Colony 1 from each transformation didn't show any amylase activity and show positive results from the Colony PCR screening, therefore 100µl of O/N liquid culture were inoculated in 50mL LB.
Mirjam
A colony PCR is done for Esta-S3 and Esta-S9. This showed correct clones for both of the ligation reactions. So an inoculation is done for these ones.
iGEM 2013 Groningen
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