Bluprint of our project

Master of Regulation: dcas9-based Multi-stage Gene Expression Regulator
Cas9 is an RNA-guided dsDNA nuclease utilized by bacteria immune system. The genetically engineered Cas9 has recently been shown to have the ability to repress or activate desired gene expression. In practical research and industrial application, we usually face the problem to express a gene at different levels, not only “on” or “off ”, a more flexible regulation method is needed.

To achieve multi-stage regulation of target genes, we further develop several dCas9 devices in which dCas9 alone or fused with omega subunit of RNAP is directed by various guide RNAs to different regions of designed double promoters. Therefore, promoters with disparate strength can be either activated or repressed respectively and multi-stage gene expression can be achieved.


Also, based on such novel technology platform, we are developing diverse applications such as a guide RNA-mediated oscillator.