Team:Groningen/28 June 2013

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Examination of all PCR products made on 27-06-2013.

The silk product is run over a 0.8% agarose gel at 90V for 24 minutes.
The signal sequences are run over a 1.5% agarose gel at 90V for 14 minutes.
The gel of the signal sequence showed nice bands.
The gel of the silk again gave the lowest band as strongest. Only at 60 degrees Celsius a smear is seen, so this is the only one that also contains the higher bands.

Made a PCR for promoter Hyperspank LacI (PCR protocol as mentioned on 25-06-2013).
The following protocol is used:
98°C, 98°C, 50°C, 72°C, 72°C, 4°C
0:30 0:10 0:25 0:45 10:00 forever