Edinburgh University

Industrial waste

We have decided to work with Bacillus subtilis as our chassis. Using it we want to solve the problem of organic and inorganic environmental pollution coming from food and beverage industry.

Our first goal will be to detect heavy metal ions. This would be followed by metal ion binding using peptides synthesised by both ribosome and a non-ribosomal peptide synthase (NRPS). To isolate the ions from the waste we will try to control biofilm formation and lock toxic metals bound to peptides in it.

To utilise the organic components of industry waste we want to produce bioethanol from it.

We hope that the project will increase the amount of useful parts available for B. subtilis (mainly the metal activated promoters and Non-ribosomal peptide synthase domains). Creation of large multi-domain enzymes and fusion proteins which is required for our project will be achieved using ground-breaking GenBrick technology which will be introduced to the iGEM community for the first time.

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