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Team:Toronto/Project/Background
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STRAINS (E. coli)
In order to assess biofilms as a system we were required to choose discrete elements, i.e. genes, to perturb from the wild type. Using the EcoCyc database as a reference we developed a regulatory map of any genes we could find related to biofilms. This map helped us identify key regulatory genes that we expected would affect the eventual phenotypic output of the biofilm; either by virtue of being a deletion strain or by the genetic element being overexpressed through our pEBS + {target gene} transformed strains. Listed below are the deletion and transformed strains we assayed along with their expected phenotypes.
BW25113 Wild Type
