Cas9 in Plants Project

A CRISPR/cas9 system is a large protein guided by a self-guiding RNA, which is capable of targeting specific DNA sequences. Cas9 has been characterized previously in bacteria and mammalian cells. Often targeted to a promoter region, Cas9 acts as highly effective gene repressing tool. The goal of the cas9 project is to make this regulatory tool available to plant genetic engineering.

Introduction

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Background

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Method

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Further Study

Due to limitations resulting from the lack of previous synthetic biology application within plants, we were unable to obtain any results. We have successfully isolated the desired parts needed for assembly of the plasmid and have the backbone with the 35s promoter left border and Nos terminator right border. Successful construct assembly of these parts is still needed for bacterial and plant transformation. Following promising transformation, fluorescence testing and characterization must be done.

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