"
Team:UCSF/Project/Accomplish
From 2013.igem.org
- We have shown that CRISPRi can be introduced to cells through bacterial conjugation. This is extremely exciting and provides the opportunity for gene expression to be altered in specific cells in a mixed population of bacteria.
- We have created a standard for creating "knock-in" cassettes and inserting DNA into the E. coli genome. Using a combination of PCR sewing and lambda red recombination, we inserted two fluorescent proteins into the E. coli genome to mark our conjugation strains.
