Team:Buenos Aires/ device
From 2013.igem.org
The Device
We developed a physical design that meets our fundamental requirements:
• easy to use
• cheap
• safe
The physical design of our device consists of a plastic support in which we will deliver the bacteria lyophilized. It will contain four different wells of bacteria: three wells will work as a standard pattern, each of them containing different amounts of arsenite physically isolated from bacteria, and a fourth well (the testing well) that will be used for sample measurement.
The usage of the device consists of:
• Collecting a sample of ground water.
• Adding the sample to the testing well and distilled water (provided with the device) to the standard pattern wells at the same time. As distilled water is added, arsenite in the standard wells dissolves and gets in contact with bacteria. At the same time, bacteria are recovered from the lyophilized state by the water added.
• Incubate for some hours, until colour differences are appreciable.
• After usage, the whole kit should be introduced in a bleach solution and disposed.
Finally, the user will be able to know if the water tested is drinkable, around the limit of arsenite allowed by the World Health Organisation or above it, by comparison with the standard pattern.
With a few simple steps, the measurement could be done, as stated in the next animation: