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Template:Kyoto/Notebook/Aug 15
From 2013.igem.org
Contents |
Aug 15
Liquid Culture
| Sample | medium |
|---|---|
| 8/10 BBa_B0034(1) | LB (+Amp) |
| 8/10 BBa_B0034(2) | LB (+Amp) |
| 8/10 BBa_R0011(1) | LB (+CP) |
| 8/10 BBa_R0011(2) | LB (+CP) |
| 8/10 BBa_P0412(1) | LB (+CP) |
| 8/10 BBa_P0412(2) | LB (+CP) |
Transformation
| Name | Sample | Competent Cells | Total | Plate |
|---|---|---|---|---|
| BBa_I0500(Pbad/araC) | 2 | 17 | 19 | LB (+Kan) |
| BBa_F1610(RBS-luxI-DT) | 2 | 17 | 19 | LB (+Amp) |
| BBa_R0061(Plux) | 2 | 17 | 19 | LB (+CP) |
| BBa_I13504(RBS-GFP-DT) | 2 | 17 | 19 | LB (+CP) |
| BBa_I0462(RBS-luxR-DT) | 2 | 17 | 19 | LB (+Amp) |
| BBa_K117000(lysis1) | 2 | 17 | 19 | LB (+CP) |
| BBa_K112806(lysis2) | 2 | 17 | 19 | LB (+CP) |
| BBa_K748002(lysis3) | 2 | 17 | 19 | LB (+CP) |
| BBa_B0015(DT) | 2 | 17 | 19 | LB (+CP) |
| BBa_Q04400(RBS-tetR-DT-Ptet) | 2 | 17 | 19 | LB (+Kan) |
| BBa_I732020(RBS-lacZα-DT) | 2 | 17 | 19 | LB (+CP) |
| BBa_J23100 | 2 | 17 | 19 | LB (+Amp) |
Miniprep
| DNA | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| BBa_B0034(1) | 84.1 | 2.11 | 2.01 |
| BBa_B0034(2) | 86.9 | 2.03 | 2.02 |
| BBa_R0011(1) | 78.3 | 2.26 | 2.02 |
| BBa_R0011(2) | 88.9 | 2.24 | 2.02 |
| BBa_P0412(1) | 15.61 | 2.10 | 1.93 |
| BBa_P0412(2) | 12.93 | 2.18 | 1.91 |
