Team:Nevada/Notebook/Month4

Revision as of 03:28, 28 September 2013 by Dordonez (Talk | contribs)

12 August 5th – August 11th

Monday

Christian and Jasmine started Top 10 Cultures for first assay.

Jon transformed LMG194 with pBAD vector containing INPN-YFP

Tuesday

Christian and Jasmine did the first lysozyme only assay.

Wednesday

Becca, Christian, and Jasmine started a culture for TOP10 cells to be used in second assay. They also purified Lyz103 and KZ144 endolysin.

Jon induced LMG194 and BL21 cells containing cell surface fluorescent markers

Thursday

Christian and Jasmine ran the Xanthomonas endolysin assay using 30 ul of purified endolysin.

Jon noted fluorescence in BL21 cells but not LMG194

Friday

Christian and Jasmine checked assay 2 results and plated contents from E11, E12, F11, and F12.

Christian, Jasmine, and Dafne completed project description.

Jon began growing large culture of Plac-INPN-YFP containing BL21 cells for assay