Exeter/16 August 2013

From 2013.igem.org

Adam + Tom

Miniprep of our complete Cph8 for inertion into CAM plasmid to be sent into the registry.

Miniprep of Complete Cph8 and Phycocyanobilin Operon (part [http://parts.igem.org/Part:BBa_K322122 BBa_K322122])

Realised we had ran out of CAM plasmid, therefore had to transform some more out of the kit plate.

Made transformations of:

TET plasmid

CAM plasmid

KAN plasmid

AMP plasmid

from the kit plates.

Checking gBlocks of Green Light module

We have three plates of our gBlocks coding for the green light module, Two show good growth, the third appears to have no growth whatsoever. Liquid cultures of all three plates were made on 15/8/13, with 16 colonies being selected from each of the three plates (48 liquid cultures in all). All liquid cultures for plate 3 (no visible cultures) had zero growth (as expected) but the liquid cultures for plates one and two were successful.

These were Miniprepped this morning, and then run on gels after