Team:Paris Bettencourt/Notebook/Drug Screening/Sunday 29th September.html

Drug Screening

Sunday 29^th September

PCR's and more!

Colony PCR on new Backbones. RFP and Biobrick verification primers This is for pSB2C and pSB3K

Colony PCR on SirA, FprA, and FdxA Biobricked parts Using Biobrick primers and SirA, FprA and FdxA primers respectfully [https://2013.igem.org/File:20130929_BioB.jpg]

Phusion PCR on Duet vectors and pSB1C3 using standard cloning primers

Gel Clean up

Digestion with NcoI and AvrII

Standard cloning of new backbones (1h ligation at 16C)

Transform into NEBTurbo

Cutting Biobrick SirA, FprA, FdxA and Linearized backbone with EcoRI and SpeI

Standard clonging of Biobrick parts (1h ligation at 16C)

Transform into NEBTurbo

Treat XbaI SpeI cut Backbone with TSAP 1h.

Standard cloning of Biobrick parts (cut with XbaI, SpeI) into TSAP'd backbone (1h @ 16C)

Colony PCR on BL21 (DE3) dcysI pACYC SirA pET FprA FdxA and BL21 AI dcysI pACYC SirA pET FprA FdxA