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From 2013.igem.org

Sander and Inne

For the silk it is decided to try a PCR with an annealing temperature of 85 and 90 degrees Celsius. we followed the usual protocol aside from the standard diluted template DNA but also tried undiluted template DNA 1, (ul in 50 ul mix for both of then) .
1 2 3 4 5 6
98 98 85/90 72 72 4
20:00 0:30 0:25 0:27 10:00 forever

Ran a gel with the 8 PCR products and the purified promoter from yesterday (as a check).

Well	1	2	3	4	5	6	7	8	9	10
	promoter	Marker	1	1.	2	2.	3	3.	4	4.

1. = duplo of sample 1

Ran the same gel again for 22 min on 100 volt to get a better picture