"

From 2013.igem.org

Mirjam
Measured the concentration of the promoter: 20,1 ng/ul.

Made a restriction digestion for the promoter and the BBa_K823023 backbone.
The following protocol is used: 159-200 ng DNA, 0.5 ul BSA, 2.5 ul EcoRI buffer, 1 ul BamHI, 0.5 ul EcoRI, adjusted to 20 ul with MQ water. The reaction is incubated for 2 hours at 37 degrees Celsius.