Exeter/7 July 2013

Making standard liquid cultures of our transformed cells

In each 10 ml Falcon, we need:

- 5 ml LB broth

- 5 ul antibiotic (in this case, all of our transformed cells are on the pSB1C3 backbone)

We used a 50 ml conical flask of LB broth which had been autoclaved, and added 50 ul of chloramphenicol to make a "stock" to be pipetted into the Falcon tubes.

We then stabbed the chosen colony with a pipette tip and ejected it into the Falcon tube.

The tubes were incubated overnight in a shaking incubator at 37°C.

Take me back to the notebook.