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From 2013.igem.org

09-09 - 15-09-13

Again, the screening-PCRs resulted in many positive colonies. After the restriction digest, many colonies were kicked out. The remaining minipreped colonies were sent in for sequencing. Yet, we did not identify any positive clone which had no mutation at the beginning of DelH (in the primer region).

Results

Colony	Alignment File	Conclusion
H33	sequencing insufficient	-
H39	sequencing insufficient	-
H40	sequencing insufficient	-
H46	sequencing insufficient	-
H51v	sequencing insufficient	-
H58	Sequencing of clone H58 with DN_07	Insertion in the primer region of DelH to Backbone
I 4E	Sequencing of clone I 4E with DN_07	Deletion of G in coding sequence
I 4H	Sequencing of clone I 4H with DN_07	Deletion of G in coding sequence
I 6B	Sequencing of clone I 6B with DN_07 Sequencing of clone I 6B with VF2	Deletion in the RBS
I 6C	Sequencing of clone I 6C with DN_07	Deletion
I 6D	Sequencing of clone I 6D with DN_07	Deletion
I 7H	Sequencing of clone I 7H with DN_07	Deletion
I 8G	Sequencing of clone I 8G with DN_07	Deletion
I 8B	-	Deletion of G in coding sequence
I 10B	-	Deletion of G in coding sequence
I 10G	-	Insertion of G in coding sequence
I 11F	-	Deletion of G in coding sequence
II 3G	-	G Deletion of the ATG (start-codon) and 3 bp later deletion of C
II 4E	-	Deletion of G in coding sequence
II 7G	-	G Deletion of the ATG (start-codon) and 3 bp later deletion of C